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. 2016 Sep 7;113(38):E5618–E5627. doi: 10.1073/pnas.1608384113

Fig. 3.

Fig. 3.

Gene-expression profiling by genome-wide array analysis. (A) Differentially expressed genes (FDR < 0.05) at different time points as identified using a multiclass rank product analysis. (B) Gene set enrichment analysis shows that proliferation is strongly induced early after stimulation of pericytes with PDFGF-BB but decreased at day 5 in comparison with 24-h stimulation. A gene set representing epithelial-to-mesenchymal transition is enriched among up-regulated genes both after 24-h and 5-d stimulation with PDFG-BB. (C) A heat map of known marker genes for pericytes, fibroblasts, mesenchymal cells, and epithelial-to-mesenchymal transition, showing that PDFGF-BB treatment of primary pericytes induced differentiation toward a fibroblast fate. (D) Heat map of stromal and mesenchymal markers, supporting the PDGF-BB–induced differentiation from pericytes toward stromal fibroblasts. (E) Gene profiling similarities between known stromal fibroblasts (S17 mesenchymal fibroblasts) and PDGF-BB–stimulated pericytes (day 5).