Fig. S4.
APC/C complexes and biochemical characterization of E2 interaction with APC/C complexes. (A) SDS PAGE gel of APC/CΔApc1−WD40 complexes. (B) SDS/PAGE analysis of UbcH10LR interactions with the APC/CΔApc1−WD40–Cdh1–Hsl1 complex (lane 5). APC/C subunits and the coeluted UbcH10LR are indicated on the right. Lanes 1-5 correspond to lanes 1, 4, 3, 5, and 7 of SDS PAGE gel (unwanted lanes 2 and 6 were removed). Lines were added to Fig. S4B to separate cropped lanes. (C) Western blot analysis of UbcH10 interactions with the ternary mutant APC/CΔApc1−WD40–Cdh1–Hsl1 complex (lane 4). The upper blot was performed with anti-Apc4 antibody to monitor the quantity of APC/C complexes used in this study, and the lower blot was performed with streptavidin to monitor the coeluted UbcH10 from the size-exclusion chromatography column.