Fig. 1.

Mnb and Wap physically interact with Cic, and Wap promotes the binding of Mnb to Cic. (A) The Cic protein interactome identified in Drosophila S2 cells (CicS) and embryos (CicV). Thick lines, highly significant interactions. A complete dataset is in Dataset S1. (B) Western blots showing coimmunoprecipitation of Cic, Mnb, and Wap in S2 cells. Endogenous dpERK is stabilized by Cic expression. (C and D) Coimmunoprecipitation of Cic, Mnb, and Wap in vivo using embryo lysates from yw (control), cic-Venus, or cic-Venus crossed with mnb-tRFP. (E) Wap is required and sufficient to bridge Cic and Mnb. (F) Cic mobility was changed when Cic was coexpressed with Wap and wild-type Mnb, but not kinase-dead Mnb (MnbKR).