Fig. 3.
Mnb reduces Cic repressor activity. (A) Diagram of the CUASC-lacZ reporter. (B) Summary diagram of expression patterns. (C–G) LacZ expression pattern resulting from C5-GAL4–directed activation of CUASC-lacZ in wing discs from control (C), UAS-cicRNAi1 (D), UAS-cic (E), UAS-mnb (F), and UAS-mnbRNAi (G) larvae. (Scale bar: 50 μm.) (H and I) Luciferase assays using CUASC-Luc reporter in S2 cells. (H) mnb, wap, and rl (ERK) are required to limit the activity of Cic. (I) Mnb and Wap reduce transcriptional repressor activity of wild-type Cic, but not of the phosphorylation site mutant, Cic-SSTS/A. n.s., not significant, *P < 0.05, **P < 0.01, ***P < 0.001, statistical significance was analyzed by using unpaired Student’s t test. Error bars represent SD.