Fig. S1.

Depletion of fluorescently tagged endogenous Mnb and Wap confirms the on-target effect of mnb-RNAi and wap-RNAi. (A–B′′) Wing discs from third instar larvae of the indicated genotypes. The en-GAL4 and hh-GAL4 drivers are expressed in the posterior compartment of wing discs (red in A’ and B’) and were used to drive the expression of UAS-mnb RNAi (A–A′′) and UAS-wap RNAi (B–B′′) respectively. Cell nuclei were detected with DAPI (blue in A and B) whereas Mnb-tRFP (gray in A′′) and Wap-Venus (gray in B′′) were detected by anti-tRFP and anti-GFP antibodies, respectively. (Scale bars: 100 µm.) Dashed lines (A′ and B′) indicate the anterior-posterior compartment boundary of the wing pouch.