Fig. S5.

Modulation of Cic repressor activity by Mnb and Wap does not require Sd and does not affect activity of ERK. (A–E) LacZ expression pattern resulting from C5-GAL4–directed activation of CUASC-lacZ in wing imaginal discs from control (A), UAS-ERK^Sem (B), UAS-wap (C), UAS-wap^RNAi (D), and UAS-sd^RNAi (E) larvae. Expression of ERK^Sem and Wap led to a broader activation of the reporter (B and C), whereas knockdown of wap resulted in lower LacZ expession, particularly in presumptive vein L5 (D). Knockdown of sd did not alter the normal pattern of expression in presumptive veins. (F–H) dpERK expression pattern in wing discs from control (F), C5-GAL4 > UAS-mnb^RNAi (G), and C5-GAL4 > UAS-wap^RNAi (H) larvae. (I) Schematic diagram of the CUASC-Luc reporter construct. (J) Dose-dependent repression of CUASC-Luc expression by Cic. S2 cells were cotransfected with the reporter CUASC-Luc, pMT-GAL4, and decreasing amounts of Cic-expressing plasmid (500 ng, 250 ng, 125 ng). The values shown are fold changes over the negative control set at 1. (Scale bar: 50 μm.)