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Loss of mnb leads to smaller brain size in larvae and pupae. (A and B) Third instar larval brains from control (w1118) (A) and mnbd419 (B) animals. (C) Quantification of the larval brain volumes in A and B (n = 2, 4). (D and E) Pupal brains from control (w1118) (D) and mnbd419 (E) animals. (F) Quantification of the pupal brain volumes in D and E (n = 4, 4). *P < 0.05. Statistical significance was analyzed by using Student’s t test. (Scale bars: 100 μm.)
