Fig. 3.

ATSs determine the spatial priority of heteromeric AMPAR assembly. (A) The dimer bands of GluA2-1_I660C were greatly reduced when coexpressed with GluA1-2. (B) GluA1-2_I664C showed constant GluA2 dimer band with GluA2-1. (C and D) GluA2-1 and GluA1-2 have the same preferred positions in tetramer as shown in A and B, with coexpression of AMPAR subunits TARP γ-2. (E–G) The glutamate-induced currents were significantly enhanced by DTT only in the GluA2-1/GluA1-2_I664C channel (n = 7). Values are means ± SEMs. ***P < 0.001 (t test). (H) Molecular surface view showing that swapping the ATSs of GluA1 and GluA2 alters the spatial assembly.