Fig. 6.

Presence of the MR on DCs impairs cytotoxicity of activated T cells in vivo. (A) The wt or MR^−/− BM-DCs were transduced with Ad-LOGs and injected into wild-type mice. After 6 d, CTLA-4 expression was monitored on SIINFEKL/Kb dextramer⁺ CD8⁺ and SIINFEKL/Kb dextramer⁻ splenic CD8⁺ T cells. The graphs depict statistical analysis (mean ± SEM) of four biological replicates. (B) Wild-type or MR^−/− BM-DCs were transduced with Ad-LOGs and injected into wild-type mice. After 5 d, mice were challenged with target (CFSE^high) and nontarget (CFSE^low) cells. After 4 h, their ratio was determined by flow cytometry. Untransduced DCs were used as controls. (C) Combined statistical analysis (mean ± SEM) of B from two independent experiments with n = 7. (D) MR expression in DCs from the liver of wild-type or MR⁻/⁻ mice. (E) GFP expression in DCs from the liver of wild-type mice infected with 10⁷ pfu of Ad-LOGs. (F) GFP expression in the liver from wild-type mice infected with 10⁷ or 10⁹ pfu of Ad-LOGs. (Scale bars: 30 μm.) (G) Proliferation of CFSE-labeled OT-I cells after incubation with sorted CD11c⁺ cells from the liver of mice infected with Ad-LOGs or control virus (Ad-LG). (H) The wt or MR⁻/⁻ mice were injected i.v. with 10⁷ pfu of Ad-LOGs. Total luciferase activity was determined after i.p. injection of 50 mM luciferin. The graph shows combined statistical analysis (mean ± SEM) from two independent experiments with n = 11. Rel., relative.