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. 2016 Sep 6;113(38):E5628–E5635. doi: 10.1073/pnas.1525078113

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Fig. S1. — Stress-induced changes of endothelial Ace in the hearts. (A) RT-qPCR quantitation of heart stress markers myh6, Myh7, Anf, Bnp, and Serca2a in the heart ventricles 7 d after sham or TAC procedure. n = 5 mice per group. P value: Student's t test. Error bar: SEM. (B) Echocardiographic measurement of FS of the LV after 7 d of TAC. (C and D) Immunostaining of Ace (brown, arrows) in mouse hearts 7 d after sham (C) or TAC (D) operation. Arrows: Ace and endothelial cells. (Scale bars, 20 μm.) (E and F) Immunostaining of Pecam in cells sorted from the hearts after sham (E) or TAC (F) operations. Original magnification: 400×. Red, Pecam; blue, DAPI. The purity of endothelial cells was >90%.