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Determination of optimum cell seeding density for the study of BM-hMSC proliferation on PPF polymer surface. The bright field microscope images of BM-hMSC-seeded PPF thin films are shown at cell densities of 10,000 (A), 25,000 (B), 50,000 (C), 100,000 (D), and, 200,000 cells/mL (E), respectively. The density of 25,000 cells/mL was chosen as initial cell density due to uniform and scattered distribution of cells (scale bar = 500 μm).
