PSC increases early engraftment in TA muscle fibers. (A)
Top, TA engraftment profiles showing counts of miniDys-GFP+ muscle fibers 7 days after transplantation with dFbs with or without PSC treatment. For each of four muscles per cohort, the number of fibers per section was counted across five sections every 700 μm through the muscles. Horizontal lines across the graph represent the average number of fibers per cohort, across all sections. Bottom, sections with maximum engraftment for each of four muscles per cohort are shown. (B) A comparison of the fiber counts in the maximum engrafted sections per muscle for each cohort. (C) A comparison of the areas in the maximum engrafted sections per muscle for each cohort. (D) A comparison of the diameters across the maximum engrafted sections per muscle for each cohort. (E) Longitudinal engraftment is shown by averaging fiber counts in the outermost sections flanking the maximum engrafted section for each cohort. (F) Higher magnification images of engrafted fibers for each cohort, of regions outlined in the bottom panels of (A). *P < 0.05. Scale bars = 400 μm. TA, tibialis anterior.