FIG. 6.

Col-I synthesis inside the HA–Ge hydrogel at the (A) zero time point, (B) after 7 days in the control with CCM flow and without phonation, and (C) after 7 days of culture in the bioreactor with phonation. Col-I and cell nuclei are stained in green and blue, respectively. The images were captured with a 20 × objective. (A−C) Scale bar is 50.0 μm. (D) A 3D-reconstructed image of Col-I and nuclei after 7 days of culture in the bioreactor. The cells were homogeneously encapsulated in the CSM, and Col-I positive staining was detected both in the vicinity of the cell membrane and throughout the CSM, 7 days after the injection. (E) Picture of the Col-I synthesis in the phonated sample with higher magnification (20 × , zoom = 6). Col-I, collagen type I; Ge, gelatin; HA, hyaluronic acid. Color images available online at www.liebertpub.com/tec