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. 2016 Sep 26;6:34299. doi: 10.1038/srep34299

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Copyright © 2016, The Author(s)

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21-day-old gerbils were inoculated with CA16-194 at 100 × LD₅₀. The graph shows the virus loads in the heart, liver, spleen, lung, kidney, brain, brainstem, spinal cord, muscle and blood from CA16-infected gerbils at 1, 2, 3 and 4 days post infection. Virus loads were assessed by real-time RT-PCR and compared with standard curves obtained from 10-fold serial dilutions of CA16-194. Results represent the mean ± standard error of the virus titer (log₁₀ TCID₅₀) per gram of tissue or per milliliter of blood from two independent experiments using three technical replicates each. ^*Significantly different from the day 1 group in each tissue (p < 0.01).