Table 1.
Primers and PCR conditions used.
Primer | Sequence | Conditions | Amplimer Size (bp) |
---|---|---|---|
GAPDH-F | 5‘-CATTGACCTCAACTACATGGT-3‘ | 94 °C × 5 min; 33 cycles: 94°C × 25 s; 53 °C × 25 s; 72 °C × 25 s; 72°C × 7 min |
130 |
GAPDH-R | 5’-TCGCTCCTGGAAGATGGTGAT-3’ | ||
HTLV-1 Tax-F | 5’-CACCTGTCCAGAGCATCAGA-3’ | 95 °C × 15 min; 45 cycles: 94°C × 30 s; 57 °C × 30s; 75 °C × 30 s; 75°C × 7 min |
264 |
HTLV-1 Tax-R | 5’-TCTGGAAAAGACAGGGTTGG-3’ |