Table 3.
Binding parameters determined from FA salt-titration assays of WT Gag proteins with non-Ψ RNA, WT Ψ, and mutant Ψ RNAs.
| RSV Gag∆PR | |||
|---|---|---|---|
| RNA a | Kd(1M) (M) b | Zeffb | Relative Specificity c |
| RSV 167 | (1.3 ± 2) × 10−1 | 7.0 ± 1.2 | 5.5 × 10−4 |
| RSV MΨ-WT | (7.1 ± 5) × 10−5 | 3.7 ± 0.5 | 1.0 |
| RSV MΨ UGCG to GAGA | (1.2 ± 0.5) × 10−5 | 2.6 ± 0.2 | 5.9 |
| RSV MΨ A197G | (4.7 ± 2) × 10−5 | 3.2 ± 0.2 | 1.5 |
| RSV MΨ GG to CC | (4.9 ± 4) × 10−4 | 4.6 ± 0.5 | 0.14 |
| RSV MΨ Triple | (6.5 ± 11) × 10−3 | 5.0 ± 1.7 | 0.011 |
| HIV-1 Gag∆p6 | |||
| TARpolyA | (5.6 ± 6) × 10−1 | 10.5 ± 1.0 | 6.4 × 10−5 |
| Ψ-WT | (3.6 ± 2) × 10−5 | 5.4 ± 0.5 | 1.0 |
| Ψ-Mut1 | (3.4 ± 5) × 10−4 | 6.7 ± 1.4 | 0.11 |
| Ψ-Mut2 | (9.5 ± 16) × 10−4 | 7.1 ± 1.4 | 0.038 |
| Ψ-Mut3 | (5.9 ± 4) × 10−5 | 5.5 ± 0.9 | 0.61 |
| Ψ-Mut4 | (2.6 ± 3) × 10−4 | 6.7 ± 2.1 | 0.14 |
| Ψ-Mut5 | (7.6 ± 6) × 10−5 | 5.9 ± 0.6 | 0.47 |
| Ψ-Mut6 | (4.6 ± 3) × 10−4 | 7.8 ± 0.8 | 0.078 |
| Ψ-Mut7 | (3.7 ± 2) × 10−5 | 5.2 ± 0.7 | 0.97 |
| Ψ-Mut8 | (2.1 ± 2) × 10−5 | 4.4 ± 1.2 | 1.7 |
a RNA concentrations were 1.5 nM for RSV Gag∆PR and 20.5 nM for HIV-1 Gag∆p6; b Kd(1M) and Zeff are defined in the legend to Table 1; c Specificity of the WT Ψ RNAs was set to 1.0 and the relative specificity of the non-Ψ or mutant Ψ RNAs was calculated as Kd(1M) (Ψ-WT)/Kd(1M) (Ψ-variant).