. 2016 Sep 26;6:33584. doi: 10.1038/srep33584

Table 6. Primers used in the study.

Primer	Target	Sequence (5′-3′)	Ann (°C)	Size (bp)	Ref.
Resistance to quinolones
gyrA-F	gyrA	CAT GCG ATG AAT GAA ATG GGA CTT TTG	55	233	19
gyrA-R	gyrA	AAA CGA CAT TCC GTG TAA CGC ATC GC	55	233	19
gyrB-F	gyrB	CTG AAG TCC GTC CAA TTG TT^*	48	634	TS
gyrB-R	gyrB	TCT TCA AAT GCT GCT TCA TT^*	48	634	TS
parE-F	parE	CAA TAC GTG ATC CTT TCG AT^*	47	564	TS
parE-R	parE	TTC CTC CTT GTG ATA TTC TG^*	47	564	TS
parC-F	parC	TCT TAT GCT AAG TGT GCA CGG A	55	349	14
parC-R	parC	TAC CAA CAG CAA TCC CTG AAG AA	55	349	14
Resistance to macrolides
rplD_F	rplD	AGA AGT CTC TGT AGC TGA GGG	49	688	TS
rplD_R	rplD	ACT GGA CTG ACA ATT ACA TCA	49	688	TS
rplV_F	rplV	CTG GAC TGA CAA TTA CAT CAT	49	702	TS
rplV_R	rplV	GGC GAC TCC AAT AGC AGA AG	49	702	TS
Resistance to macrolides and amphenicols
23S_rRNA_F	23S rRNA	AGT GAA ATT GAA TTC CCC	46	780	TS
23S_rRNA_R	23S rRNA	GGA ATA CTC GTT TTC AGG T	46	780	TS
Resistance to rifamycins
rpoB-F	rpoB	GAT GAT ATC GAC AAT CTT GGT A^**	49	818	TS
rpoB-R	rpoB	GCA GCA CCT GAA TCA CGA GCC	49	818	TS
Bacterial identification
16SBartonella-F	16S rRNA	CCT TCA GTT MGG CTG GAT C	55	438	59
16SBartonella-R	16S rRNA	GCC YCC TTG CGG TTA GCA CA	55	438	59

TS. This Study.

^*These primers are modifications of those described by Angelakis et al.⁴³.

^**This primer is a modification of the BarpoBF primer designed by Biswas et al.¹⁸.