Figure 4. Direct binding of Ska3_C-term to Ska1 and microtubules is required for maintaining kinetochore-microtubule attachments and timely anaphase onset.

(a,b) Box-and-whisker plot showing the elapsed time (min) between nuclear envelope breakdown (NEBD) and anaphase onset/death for individual cells. The total number of cells (n) from three independent experiments is given above each box. Lower and upper whiskers represent 10^th and 90^th percentiles, respectively. Table summarizing information from the live cell experiments shown below regarding the average time between NEBD to anaphase onset/death and the percentage of cells dying in mitosis. (c) Representative stills from time-lapse video microscopy experiments illustrating mitotic progression of HeLa S3 cells stably expressing H2B-GFP, treated as in (a,b). Time in hr:min is indicated. T = 0 was defined as the time point at which NEBD became evident. Scale bar, 10 μm.