Figure 1. Inhibition of different HDAC subtypes by individual inhibitors in neonatal rat cardiomyocytes.

(A–D) Active recombinant HDAC2, 4, 7 or 8 was incubated with a specific unique fluorogenic substrate in the presence or absence of the HDAC inhibitor, PBA (A), BS (B), TSA (C) or VAL (D), at different doses. The system’s fluorescence intensity was measured using a fluorescence reader after adding Lysine Developer. Data are expressed as mean ± SEM; n = 3; *P < 0.05 vs. the corresponding baseline of each HDAC subtype.