Table 2. Description, expression and putative target genes of known miRNAs whose expression were significantly down-regulated in roots of both Guiyan 1 (G) and Yunyan 2 (Y) treated with 50 μM Cd for 5 days.
| Family name | miRNA name | Sequence | Length (nt) | TPMa |
Fold changeb |
Target gene | Annotation | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| G−Cd | G + Cd | Y−Cd | Y + Cd | G | Y | ||||||
| miR166 | nta-miR166a | UCGGACCAGGCUUCAUUCCCC | 21 | 49481.3 | 17265.8 | 51937.0 | 6379.0 | −1.5 | −3.1 | TC126889 | PHAVOLUTA-like HD-ZIPIII protein (N. sylvestris) |
| nta-miR166b | UCGGACCAGGCUUCAUUCCCC | 21 | 49978.8 | 17303.7 | 51466.5 | 6467.4 | −1.5 | −3.0 | |||
| nta-miR166c | UCGGACCAGGCUUCAUUCCCC | 21 | 49723.1 | 17629.6 | 51621.0 | 6216.5 | −1.5 | −3.1 | |||
| nta-miR166d | UCGGACCAGGCUUCAUUCCCC | 21 | 49377.6 | 17569.0 | 51649.1 | 6725.6 | −1.5 | −3.0 | |||
| nta-miR166e | UCGGACCAGGCUUCAUUCCCC | 21 | 49636.7 | 17318.9 | 52232.0 | 6618.6 | −1.5 | −3.0 | |||
| nta-miR166f | UCGGACCAGGCUUCAUUCCCC | 21 | 50676.6 | 18054.1 | 52246.0 | 6390.7 | −1.5 | −3.1 | |||
| nta-miR166g | UCGGACCAGGCUUCAUUCCCC | 21 | 50369.1 | 17152.1 | 53095.9 | 6446.0 | −1.6 | −3.1 | |||
| nta-miR166h | UCGGACCAGGCUUCAUUCCCC | 21 | 49450.2 | 17091.5 | 53404.9 | 6560.9 | −1.5 | −3.1 | |||
| miR6149 | nta-miR6149a | UUGAUACGCACCUGAAUCGGC | 21 | 23011.6 | 8535.6 | 24061.7 | 5060.5 | −1.5 | −2.3 | FS390832 | TCTR2 protein (S. lycopersicum) |
aTPM value indicates the expression level of miRNA; TPM value = counts of this miRNA/ total counts of this sample × 1000000.
bFold change (Cd vs control) is log2N, log2N ≥ 1.5 are up-regulated, between 0 < |log2N| < 1.5 are unchanged and log2N ≤ −1.5 are down-regulated, p < 0.01. G–Cd, G + Cd, Y − Cd and Y + Cd correspond to hydroponically grown tobacco of the cultivars Guiyan 1 and Yunyan 2 grown in basic nutrition solution (BNS) or BNS + 50 μM Cd.