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. 2016 Sep 23;7:12852. doi: 10.1038/ncomms12852

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Paired IDC and DCIS LCM samples were analysed using DigiWest.(a) Her2-stained sections, paired IDC and DCIS from three patients. (b) Her2 signals for the LCM samples on DigiWest. (c) Volcano plot representing the mean IDC/DCIS ratio for all measured analytes versus significance. P values were derived from t-tests with Welch approximation. The data contained in this volcano plot are based on quantified signals obtained from an equivalent to about 1,000 western blot lanes.