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. 2016 Sep 22;7:12860. doi: 10.1038/ncomms12860

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Copyright © 2016, The Author(s)

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Isolates were grown in marine broth with added alginate oligosaccharides to induce enzyme expression. The cells were lysed to determine the total, cell associated alginate lyase activity by measuring the increase of absorption at 235 nm with alginate as enzyme substrate. The alginate lyase activity of each strain was normalized against the optical density of the respective cell culture measured at 600 nm. The experiment was carried out in triplicate and the error bars display the standard deviation of the mean.