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. 2016 Sep 26;6:34163. doi: 10.1038/srep34163

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Flag-MEF2C and EGFP-G9a were transfected in HEK293T cells which were subsequently treated with UNC0638 for 48 hr. MEF2C was immunoprecipitated with anti-Flag agarose beads and immunoblotted with anti-Flag and anti-GFP antibodies. 10% lysates (Input) were probed for G9a and MEF2C expression. β-actin was used as a loading control. (b) MEF2C was co-transfected with Flag-G9a or Flag-G9a ∆SET in HEK293T cells. G9a was immunoprecipitated using anti-Flag agarose beads and probed for association with MEF2C. 10% lysates were analyzed for G9a and MEF2C expression. (c) Endogenous G9a was immunoprecipitated from C2C12 lysates and probed for association with endogenous MEF2C. IgG was used as a negative control. Input shows expression of G9a and MEF2C in the lysates. (d) Schematic representation of full length MEF2C (FL), MEF2C lacking the MADS domain (∆MADS), and MEF2C with a truncated C-terminus (N177). Numbers indicate amino acid residues on MEF2C. EGFP-G9a was co-expressed with FL, ∆MADS, and N177. MEF2C was immunoprecipitated with anti-Flag agarose beads and lysates were probed for association with G9a using anti-GFP antibody. The expression of full length and MEF2C deletion mutants was analyzed in immunoprecipitates with anti-Flag antibody. Input refers to G9a and MEF2C expression in the lysates. β-actin was used as a loading control. Asterix indicates specific MEF2C bands.