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. 2016 Sep 26;6:33878. doi: 10.1038/srep33878

Table 2. Primers used in 5′RACE.

Primer’s name Sequence Usage
MG1-Hinge_708-683R ACCACAATCCCTGGGCACAATTTTCT RT for IgG1 heavy chain
MG1-PCR_438-409R AGGGGCCAGTGGATAGACAGATGGGGGTGT 1st PCR for IgG1 heavy chain
MVH2R ATAGACAGATGGGGGTGTCGTTTTGGC 2nd PCR for IgG1 heavy chain
MK-Edge_711-685R CTCATTCTTGTTGAAGCTCTTGACAAT RT for Kappa chain
MK-PCR_435-403R GGATGGTGGGAAGATGGATACAGTTGGTGCAGC 1st PCR for Kappa chain
Adaptor + G GGCCACGCGTCGACTAGTACGGGGGGGGGG 1st PCR
Adaptor GGCCACGCGTCGACTAGTAC 2nd PCR

RT: reverse transcript.