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. 2015 Jul 6;65(10):1744–1753. doi: 10.1136/gutjnl-2014-307856

Figure 1.

Figure 1

HCV can infect HIL mice leading to liver inflammation and liver injury. (A and B) Ten-week-old HIL mice were mock-infected or HCV-infected. (A) Human epidermal growth factor receptor+ (hEGFR+) cells were purified from mouse livers at 2 and 5 weeks post-infection and analysed for HCV RNA by RT-PCR (n≥5 mice per group). In one group of mice, HCV infection was performed with T cell depletion and analysis was performed at 5 weeks post-infection (indicated by #). Values from each mouse are plotted as symbols, and the average values for each group are plotted as solid lines. (B) Staining of dsRNA (in red) and CK18 (in green) in the hEGFR+ cells purified from HIL mice at 2 and 5 weeks post-infection. (C) Livers were harvested and paraffin sections were prepared 5 weeks post-HCV infection (n=5 mice per group). Representative stains for DAPI (blue), anti-HCV core (green) and antihuman albumin (hALB) (red) are shown. (D and E) Ten-week-old HIL mice were infected with HCV or mock-infected for 0, 1, 3, 5 and 9 weeks (n=5 mice per group). Livers and sera were harvested for histology and alanine aminotransferase (ALT) assay. (D) Representative liver H&E stains of mock-infected and HCV-infected mice from different time points are shown. (E) Serum ALT levels in HIL mice that were mock-infected or HCV-infected from different time points are represented. Data represent mean±SEM.