FIG. 3.

Mice colonization with WT X47-2AL and its isogenic amiA mutants after 3, 15, and 30 days of infections (A), and with WT B128 and its isogenic amiA mutants 15 days after infection (B). For each experiment, we used an even mixture of three independent clones of the amiA mutants. Since the amiA mutant chains, we considered it was plausible that we were not able to detect colonization of the mutant using a low infectious dose (represented with gray circles). Therefore, a higher dose was also tested (represented with dark triangles). The amiA mutant was still unable to colonize C57/BL6J mice (ND = non detectable). The data were submitted to a Mann–Whitney test (**p < 0.01).