FIG. 4.

Membrane localization of hMCT8 demonstrated by confocal microscopy in Mct8KO mice injected with AAV9-ShMCT8 IV and ICV (for dose, see legend to Fig. 3). Representative images showing immunopositive signal for hMCT8 (in red) and nuclei stained with DAPI (in blue) in the choroid plexus (A, B), cerebral cortex (C, D) and CA1 region of the hippocampus (E, F) of Mct8KO mice injected with AAV9-ShMCT8 IV (A, C, E) or ICV (B, D, F). hMCT8 was neatly localized at the apical membrane of the choroid plexus of IV-injected mice (A). A similar localization was observed in only few cells of ICV injected animals (B). hMCT8 was localized in the membranes (see white arrows) of cortical and hippocampal neurons of animals injected either IV or ICV (C–F). However, a greater amount of hMCT8 was present in the cytoplasm of animals injected ICV. (A, B) Scale bar = 20 μm; (C–F) scale bar = 10 μm. Color images available online at www.liebertpub.com/thy