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. 2016 Sep 1;22(6):499–509. doi: 10.1089/mdr.2016.0128

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© Regina Kilian, et al., 2016; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 2. — Analysis of balhimycin production, muropeptide composition, and PG precursors in A. balhimycina WT (1), A. balhimycina ΔvnlR_Ab (2), A. balhimycina [vnlR_Ab] (3), and A. balhimycina ΔvnlR_Ab [vnlR_Ab] (4). (A) Production of balhimycin measured by HPLC (n = 5). (B) HPLC/MS chromatogram of the muropeptides (positive mode). The first bracket embraces the peaks representing muropeptide monomers, the second the muropeptide dimers. (C) Extracted ion chromatograms of the negative mode from the PG precursors isolated from cells grown in R5 (balhimycin production) and in TSB (no balhimycin production). d-Lac, Pentapeptide precursors ending on d-Ala-d-Lac 1194 m/z at retention time ∼18 min. d-Ala, Pentapeptide precursors ending on d-Ala-d-Ala 1193 m/z at retention time ∼12 min. HPLC, high-performance liquid chromatography; MS, mass spectrometry; PG, peptidoglycan; WT, wild type.