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RT-PCR analyses of vanHAXAb and vanYAb in A. balhimycina WT, A. balhimycina ΔvnlRAb, and in A. balhimycina WT overexpressing vnlRAb (WT [vnlRAb]). RNA was isolated at different time points (15/39/63 hr) from the three strains cultivated in balhimycin production medium R5. sigB: transcription of the housekeeping gene sigB. vanHAXAb and vanYAb: transcription of vanHAXAb and vanYAb. For PCR, genomic DNA (gDNA) was used as positive control.
