Table 1.
Bacterial Strains Used in This Study
| Relevant feature(s) | References | |
|---|---|---|
| Strains | ||
| Streptomyces coelicolor A3(2) | ||
| M600 | SCP1- SCP2- | 30 |
| M600 ΔvanRSSc (J2301) | vanRSSc deletion mutant | 6 |
| M600 ΔvanRSSc [vnlRSAb] | ΔvanRSSc complemented with pRM4vnlRSAb | This study |
| M600 ΔvanRSSc [vnlRAb] | ΔvanRSSc complemented with pRM4vnlRAb | This study |
| M600 ΔvanRSSc [vnlSAb] | ΔvanRSSc complemented with pRM4vnlSAb | This study |
| M600 ΔvanRSSc [vnlRAbD51A] | ΔvanRSSc complemented with pRM4vnlRAbD51A | This study |
| Amycolatopsis balhimycina DSM 5908 | ||
| A. balhimycina WT DSM 5908 | Wildtype | 24 |
| A. balhimycina ΔvnlRAb | vnlRAb deletion mutant | 27 |
| A. balhimycina [vnlRAb] | Overexpression of vnlRAb in A. balhimycina, using pRM4vnlRAb | This study |
| A. balhimycina ΔvnlRAb [vnlRAb] | ΔvnlRAb complemented with pRM4vnlRAb | This study |
| A. balhimycina ΔvnlSAb | vnlSAb deletion mutant | This study |
| Escherichia coli | ||
| XL1-blue | recA1; endA1; gyrA96; tji-1; hsdR17; supE44; relA1; lac [F'proAB, lacqZΔM15Tn10(tetr)] | 28 |
| ET 12567 pUZ8002 | pUZ8002; kanr | 34 |
| ET 12567 | F-; dam13::Tn9; dcm-6; hsdM; hsdR; recF143; zjj201::Tn10; galK2; galT22; ara14; lacY1; xyl15; leuB6; thi1; tonA31; rpsL136; hisG4; tsx78; mtli; glnV44 | 29 |
| Plasmids | ||
| pRM4 | pSET152 ermEp*, RBS, Φ31 attP-int-derived integration vector | 31 |
| pRM4vnlRSAb | Expression plasmid for vnlRSAb | This study |
| pRM4vnlRAb | Expression plasmid for vnlRAb | This study |
| pRM4vnlSAb | Expression plasmid for vnlSAb | This study |
| pRM4vnlRAbD51A | Expression plasmid for vnlRAb, | This study |
| Exchange of Asp at position 51 with Ala | ||
| pSP1 | Inactivation vector in A. balhimycina | 32 |
| Erythromycin and ampicillin resistance | ||
| pSPΔvnlSAb | pSP1 carrying a 1579 bp upstream and a 1509 bp downstream fragment of vnlSAb | This study |
WT, wild type.