Table 2.
Primers Used in This Study
| Primer | Sequence | Relevant feature(s) | References | |
|---|---|---|---|---|
| 1 | vnlRS-compl.1 | CATCGGCATATGCGCGTGCTGATCGTCGAG | Cloning of vnlRSAb | This study |
| 2 | vnlRS-compl. 2 | GAATTCCTGCGCGACTCCAGCGTTTCTCAGCGGAAG | ||
| 3 | vnlR-over | GAATTCAGGCGTAGCTGAGG | Cloning of vnlRAb | This study |
| 4 | vnlS-cloning | ATCATATGAGCGTCCGCCTCAAAC | Cloning of vnlSAb | This study |
| 5 | vnlRD51A1 | GAATATCCCGGgCGAGGACGG | Recombinant primers for aa exchange | This study |
| 6 | vnlRD51A2 | CCGTCCTCGcCCGGGATATT | ||
| 7 | vnlSdelFrg1for | TTAGAATTCGATTGTCCGCGAGAAATG | Cloning of vnlSAb upstream region | This study |
| 8 | vnlSdelFrg1rev | TAATCTAGACCCGGCCGCTCTGTC | ||
| 9 | vnlSdelFrg2for | TTATCTAGACCGGCCGGGTCACCTC | Cloning of vnlSAb downstream region | This study |
| 10 | vnlSdelFrg2rev | ATTGCATGCGGGCGCAAGTGAGTTTCGGTCATCG | ||
| 11 | pSETerme rev | ATGCTAGTCGCGGTTGA | Integration of Plasmid and Insert | This study |
| 12 | attBli-fwd | TTCTGGAAATCCTCGAAGGC | Integration of plasmid through ΦC31 | |
| 13 | attPint-rev | TGTGCATGCGCCCACGAATG | ||
| 14 | ery for | AAGGGAGAAAGGCGGACAGG | Proof of erythromycin resistance cassette | This study |
| 15 | ery rev | GTCGCTTCTGCGCAAGTACC | ||
| 16 | vnlSproof for | TGCTCGAAGTCCTCGTTTCC | Integration and deletion verification | This study |
| 17 | vnlSproof rev | GCAAGTACGTGAGCGATCAG | ||
| 18 | vanSSc_RT_1 | CTCCAACTGACCACGAACCT | RT-PCR analysis in S. coelicolor M600 | This study |
| 19 | vanSSc_RT_2 | GGTCGGTGTGTATGCGTTC | ||
| 20 | vanRSc_RT_1 | TGCTGAGTGTCAACGCCTAC | ||
| 21 | vanRSc_RT_2 | CGAACTGCTTCCTGGTCAAC | ||
| 22 | vanASc_RT_1 | ACCGTGACAGGAGACGAGAC | ||
| 23 | vanASc_RT_2 | CTGGTGGATCCGGAAGAAT | ||
| 24 | hrdBSc_RT_1 | TGACCAGATTCCGGCCACTC | This study | |
| 25 | hrdBSc_RT_2 | CTTCGCTGCGACGCTCTTTC | ||
| 26 | sigB for | CGTAGGTCGAGAACTTGAAC | RT-PCR analysis in A balhimycina DSM5908 | 41 |
| 27 | sigB rev | GTGTCTACCTCAACGGTATC | ||
| 28 | vanH1 | GGGACAAGCCCATCAAGAAC | 27 | |
| 29 | vanA2 | GAGCGGACTTGACGGAGATG | ||
| 30 | vanY_RT_fwd | TCGGCACGAGGATTG | ||
| 31 | vanY_RT_rev | TTCACGCACAGTTCG |
RT-PCR, reverse transcriptase polymerase chain reaction.