Fig. 5.

ANGPTL4 lowers the amount of LPL on the adipocyte cell surface. A: Western blot of cell lysates of gWAT explants from Angptl4^−/− and wild-type mice incubated in the absence or presence of heparin (50 IU/ml) for 3 h. Western blots were probed with antibodies against LPL and HSP90 (as loading control). B: Western blot of cell lysates of adipocytes that had been differentiated from stromal vascular fractions of WAT from Angptl4^−/− and wild-type mice and incubated in the absence or presence of heparin (10 IU/ml) for 20 min. Western blots were probed with antibodies against LPL and HSP90 (as loading control). C: Angptl4 and Lpl mRNA levels in gWAT explants from Angptl4^−/− and wild-type mice. D: Angptl4 and Lpl mRNA levels in adipocytes that had been differentiated from stromal vascular fractions of WAT from Angptl4^−/− and wild-type mice. EndoH-resistant LPL (complex oligosaccharides; Golgi and cell surface LPL) is indicated with R; EndoH-sensitive LPL (high-mannose oligosaccharides, ER LPL) is indicated with S.