Fig. 6.

ANGPTL4 lowers LPL secretion. A: Western blot of media of gWAT explants from Angptl4^−/− and wild-type mice treated in the absence or presence of heparin (50 IU/ml) for 3 h. Western blots were probed with antibodies against LPL and ADIPOQ. Coomassie blue staining was used to assess loading. B: Western blot of media of adipocytes that had been differentiated from stromal vascular fractions of WAT from Angptl4^−/− and wild-type mice and incubated in the absence or presence of heparin (Hep.) (10 IU/ml) for 20 min. Western blots were probed with antibodies against LPL and ADIPOQ. Coomassie blue staining was used to assess loading. C: Western blot of media of adipocytes that had been differentiated from stromal vascular fractions of WAT from Angptl4^−/− and wild-type mice. Cells were pretreated for 20 min with heparin (10 IU/ml), washed with PBS, and incubated with heparin (10 IU/ml) for the indicated times. Western blots were probed with antibodies against LPL and ADIPOQ. EndoH-resistant LPL (complex oligosaccharides; Golgi and cell surface LPL) is indicated with R; EndoH-sensitive LPL (high-mannose oligosaccharides, ER LPL) is indicated with S.