Fig. 8.

Physiological regulation of ANGPTL4 expression affects levels of EndoH-resistant LPL in vivo. A: Western blot of gWAT lysates of Angptl4^−/− and wild-type mice, as analyzed by SDS-PAGE and native PAGE. Western blots were probed with antibodies against LPL and HSP90. Coomassie blue staining was used to assess loading. B: Western blot of EndoH-treated and PNGase-treated gWAT lysates of Angptl4^−/− and wild-type mice. Western blots were probed with antibodies against LPL and HSP90 (as a loading control). C: Western blot of EndoH-treated gWAT lysates prepared from ad libitum-fed, overnight-fasted, or refed Angptl4^−/− and wild-type mice. The tissue samples were taken from an experiment described earlier (13). Western blots were probed with an antibody against LPL; Coomassie blue staining was used to assess loading. D: Western blot of gWAT lysates prepared from ad libitum fed, overnight-fasted, or refed wild-type mice. The tissue samples were taken from an experiment described earlier (13). Western blots were probed with an antibody against ANGPTL4; Coomassie blue staining was used to assess loading. E: Western blot of EndoH-treated gWAT lysates from Angptl4^−/− and wild-type mice that were either not perfused or perfused with PBS containing heparin (50 IU/ml). Western blots were probed with antibodies against LPL and HSP90 (as a loading control). F: Western blot of EndoH-treated gWAT lysates prepared from fed and overnight-fasted Angptl4^−/− and wild-type mice that had been given an intravenous injection of heparin (100 IU/kg). Western blots were probed with antibodies against LPL and HSP90 (as a loading control). EndoH-resistant LPL (complex oligosaccharides; Golgi and cell surface LPL) is indicated with R; EndoH-sensitive LPL (high-mannose oligosaccharides, ER LPL) is indicated with S.