Fig. 9.

Levels of EndoH-resistant LPL are inversely related to Angptl4 expression. A: Western blot on 0.75 μl of plasma from Angptl4^−/− and wild-type mice. Western blots were probed with an antibody against LPL; Coomassie blue staining was used to assess loading. B: Western blot of EndoH-treated WAT lysates prepared from Angptl4^−/−, Angptl4⁺/^-, and wild-type mice. Western blots were probed with an antibody against LPL; Coomassie blue staining was used to assess loading. C: Western blot of EndoH-treated WAT lysates prepared from Angptl4^−/−, wild-type, and Angptl4-Tg mice. Western blots were probed with an antibody against LPL; Coomassie blue staining was used to assess loading. D: Western blot of EndoH-treated lysates of 3T3-F442a adipocytes that had been treated with rosiglitazone (10 μM) or DMSO control for 6 h. Western blots were probed with antibodies against LPL, ANGPTL4, and HSP90 (as a loading control). E: Western blot of EndoH-treated lysates of 3T3-L1 adipocytes that had been treated with rosiglitazone (10 μM) or DMSO control for 6 h. Western blots were probed with antibodies against LPL, ANGPTL4, and HSP90 (as a loading control). EndoH-resistant LPL (complex oligosaccharides; Golgi and cell surface LPL) is indicated with R; EndoH-sensitive LPL (high-mannose oligosaccharides, ER LPL) is indicated with S.