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Diagrammatic representation of the PUFA synthase constructs for E. coli expression. Black squares represent ribosome binding sites, T7-P represents T7 promoter, and T7-T represents T7 terminator. A: Three different plasmids were used to express three ORFs of the PUFA synthase ORFs in E. coli. B: One plasmid was used to express three ORFs of the PUFA synthase as one operon in E. coli.
