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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Macromol Biosci. 2015 Dec 28;16(4):496–507. doi: 10.1002/mabi.201500361

Figure 4. Proteolytic degradation of visible light-cured mixed-mode PEG hydrogel properties.

Figure 4

(A) Schematic of a protease sensitive peptide crosslinker (CGGYC) within a thiol-acrylate hydrogel. (B) Mass change of 10wt% PEGDA thiol-acrylate hydrogels induced by exogenous chymotrypsin (2mg/mL) treatment. Total di-thiol crosslinkers (CGGYC and DTT at indicated percentage) was 7.5mM. Other conditions: 0.1vol% NVP, 0.1mM eosin-Y, and 5-min visible light exposure. (C) Exogenous chymotrypsin (2mg/mL) induced mass change of 10wt% PEGDA thiol-acrylate hydrogels crosslinked with different NVP contents. Other conditions: 7.5mM CGGYC; 0.1mM eosin-Y, and 5-min visible light exposure. (D) Mass change of 10wt% PEGDA thiol-acrylate hydrogels induced by exogenous chymotrypsin treatment. Other conditions: 7.5mM CGGYC, 0.1vol% NVP, 0.1mM eosin-Y, and 5-min visible light exposure. (E) Mass change of 4wt% PEG4A thiol-acrylate hydrogels induced by exogenous chymotrypsin treatment. Other conditions: 4mM CGGYC, 0.1vol% NVP, 0.1mM eosin-Y, 5-min visible light exposure.