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. 2016 Jul 14;17(9):925–934. doi: 10.1080/15384047.2016.1210728

Figure 2.

Figure 2.

Berberine synthesized with lapatinib reversed lapatinib resistance in both BT-474LapR cells and AU-565LapR cells. The cells were treated with lapatinib alone, berberine alone and a combination of lapatinib and berberine. (A) An MTT assay was used to demonstrate sensitization of berberine to lapatinib in BT474LapR cells and AU-565LapR cells. The cells were treated various concentrations of berberine (0-16 μM) synthesized with lapatinib (2 μM). (B) Flow cytometry was used to demonstrate sensitization of berberine to lapatinib. The cells were treated with lapatinib (2 μM), berberine (2 μM) and both (L+B), and the control group was untreated. The right figure shows the relative apoptosis rate of cells. *p < 0.05, **p < 0.01. (C) Colony formation was used to prove that berberine could sensitize lapatinib-resistant cells to lapatinib. The cells were treated with lapatinib (2 μM), berberine (2 μM) and a combination of both (L+B), and the control group was untreated. The right figure shows the relative colony formation rate of cells. *p < 0.05, **p < 0.01.