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. 2016 Sep 9;6:32960. doi: 10.1038/srep32960

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Copyright © 2016, The Author(s)

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(a) Inhibition of PPP1R14a expression in HEK 293T -cells constitutively expressing HMGB4-EGFP. Three independent repeats were analyzed by comparing representative EGFP expressing control and HMGB4-EGFP expressing cells. Results from representative analysis are shown. (b) Mouse E14 cortical neurons and C2C12 myoblast cells, both expressing endogenous HMGB4 mRNA, were treated with HMGB4 Vivo-Morpholino to downregulate translation of HMGB4. The expression levels of PPP1R14a were analyzed with qPCR. The levels of the PPP1R14a transcript in HMGB4 Vivo-Morpholino -treated cells were normalized to the PPP1R14a transcript levels in control Vivo-Morpholino -treated cells (*p < 0.05, ± SEM; n = 6 in C2C12 experiment; n = 18 in neuron experiment).