Table 2. Neuronal differentiation qPCR-array of HMGB4 shRNA -infected NTERA-2 cl. D1 cells.
| GENE | CELL TYPE/FUNCTION | AVERAGE (±SE MEAN) | P | |
|---|---|---|---|---|
| DOWNREGULATED | NEUROD1 | TRANSCRIPTION FACTOR ENHANCING NEURONAL DIFFERENTIATION | 0.071 ± 0.017 | 0.014 |
| MBP | OLIGODENDROCYTE | 0.114 ± 0.026 | 0.000001 | |
| FABP7 | ASSOCIATED TO RADIAL GLIA AND NEUROGENESIS | 0.123 ± 0.025 | 0.000001 | |
| SLC32A1 | GABA NEURON | 0.132 ± 0.042 | 0.01 | |
| HMGB4 | NEURON | 0.237 ± 0.052 | 0.0001 | |
| NR4A2 | DOPAMINERGIC NEURON | 0.301 ± 0.073 | 0.003 | |
| NCAM1 | MATURE NEURON | 0.357 ± 0.084 | 0.035 | |
| GFAP | ASTROCYTE | 0.377 ± 0.087 | 0.012 | |
| CNP | OLIGODENDROCYTE | 0.606 ± 0.011 | 0.011 | |
| UNREGULATED | MAPT | NEURON | 0.327 ± 0.071 | 0.15 |
| S100B | MATURE NEURON, ASTROCYTE | 0.438 ± 0.12 | 0.13 | |
| PAX6 | RETINAL PROGENITOR | 0.449 ± 0.094 | 0.19 | |
| GAD2 | GABA NEURON | 0.478 ± 0.15 | 0.15 | |
| FOXG1 | MOTONEURON | 0.626 ± 0.19 | 0.21 | |
| OLIG2 | OLIGODENDROCYTE PROGENITOR | 1.035 ± 0.22 | 0.92 | |
| NKX2-2 | OLIGODENDROCYTE PROGENITOR, OLIGODENDROCYTE | 1.052 ± 0.17 | 0.85 | |
| SLC1A2 | GLUTAMATERGIC NEURON | 1.118 ± 0.31 | 0.80 | |
| SLC1A3 | GLUTAMATERGIC NEURON | 1.217 ± 0.24 | 0.55 | |
| EMX1 | NEURON | 1.644 ± 0.44 | 0.26 | |
| UPREGULATED | ASCL1 | NEUROBLAST FORMATION | 3.917 ± 1.40 | 0.01 |
Gene expression was analyzed with neuronal differentiation qPCR array and with HMGB4 qPCR, and the expression levels of HMGB4 shRNA -infected cells were normalized to the expression levels of control shRNA -infected cells. Average fold changes in gene expression are shown (n = 4 in neuronal differentiation qPCR array, and in HMGB4 analysis n = 3 for controls and n = 12 for HMGB4).
One-way ANOVA was used to calculate p-values.