Figure 3. sFlt3L and poly-ICLC induce a Batf3-dependent increase in the numbers of tumor-antigen-specific TILs expressing CD137 and PD-1.
WT or Batf3-/- mice were inoculated with B16-OVA melanoma cells on day 0, concomitant with hydrodynamic gene transfer of sFlt3L or control empty plasmid. On day 7, tumors were injected with poly-ICLC or control. Tumors were retrieved and TILs analyzed on day 10. (A) H2Kb-OVA257-264 tetramer staining in CD8+ TILs. Left: Representative plots. Right: Graphs corresponding to a representative experiment (n = 3) (B) Surface CD137 and PD-1 immunostaining in CD8 TILs. (C) PD-1 and CD137 surface immunostaining in SIINFEKL tetramer+ gated T cells. One-way ANOVA with Bonferroni post-hoc test, * p < 0.05; ** p < 0.01; *** p < 0.001.