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One-pot two-enzyme (OP2E) synthesis of sialosides containing Neu4,5Ac2 (1) or Neu4Ac5Gc (2). Enzymes: NmCSS, Neisseria meningitidis CMP-sialic acid synthetase; PmST3, Pasteurella multocida α2–3-sialyltransferase 3. The pH of the reaction was controlled at 7.1 to minimize de-O-acetylation during the enzymatic reaction. Yields were calculated for purified products based on the amounts of acceptors (limiting reagents) used.
