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. Author manuscript; available in PMC: 2017 Sep 28.
Published in final edited form as: Org Biomol Chem. 2016 Aug 22;14(36):8586–8597. doi: 10.1039/c6ob01706a

Scheme 4.

Scheme 4

Sequential one-pot multienzyme (OPME) synthesis of LNnTβProN3 (23). Enzymes: BLNahK or NahK_ATCC55813, Bifidobacterium longum N-acetylhexosamine-1-kinase; PmGlmU, Pasteurella multocida N-acetylglucosamine uridyltransferase; PmPpA, Pasteurella multocida inorganic pyrophosphatase; NmLgtA, Neisseria meningitidis β1–3-N–acetylglucosaminyltransferase; EcGalK, Escherichia coli galactokinase; BLUSP, Bifidobacterium longum UDP-sugar pyrophosphorylase; NmLgtB, Neisseria meningitidis β1–4-galactosyltransferase.