Table 1.
One-pot two-enzyme (OP2E) preparative-scale synthesis of α2–3-linked 4-O-acetylated sialosides. Yields were calculated for purified products based on the amounts of acceptors (limiting reagents) used.
| Entry | Acceptor | Donor | Sialosides(Neu4,5Ac2/Neu4Ac5Gca3OR) | yield |
|---|---|---|---|---|
| a |
|
|
|
82% |
| b |
|
1 |
|
78% |
| c |
|
1 |
|
65% |
| d |
|
1 |
|
31% |
| e |
|
1 |
|
88% |
| f |
|
1 |
|
85% |
| g |
|
1 |
|
86% |
| h |
|
1 |
|
80% |
| i |
|
1 |
|
81% |
| j |
|
1 |
|
74% |
| k |
|
1 |
|
81% |
| l | 17 |
|
|
67% |
| m | 18 | 2 |
|
71% |
| n | 19 | 2 |
|
65% |
| o | 20 | 2 |
|
56% |
| p | 21 | 2 |
|
51% |
| q | 22 | 2 |
|
52% |
| r | 23 | 2 |
|
62% |