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. Author manuscript; available in PMC: 2017 Sep 9.
Published in final edited form as: Xenotransplantation. 2016 Sep 9;23(5):381–392. doi: 10.1111/xen.12260

Figure 4.

Figure 4

Quantitative analysis of sialic acids in xenogenic cardiac tissues by DMB-HPLC. Neu5Ac, Neu5Gc and their O-Acetylated derivatives were quantified by DMB-HPLC in native porcine aortic valve cusp (A), pulmonary valve cusp (B), and pericardium (C), and bovine pericardium (D). 2 μl of tissue homogenates were acid hydrolyzed to release Sia followed by DMB-labeling and HPLC analysis. Sia content was quantified according to a standard curve of purified Neu5Ac. Percentage of each Sia from the total detected is indicated on the charts. All detected O-Acetylation types were summarized (at least two different samples from each tissue were tested, data represent at least two independent experiments; mean ± SEM).