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Nanometry of single kinesin molecules. (a) The optical trapping nanometry system (not to scale). The kinesin-coated bead captured by the optical tweezers was used as a probe to measure the kinesin displacement. (b) A typical displacement record by a single kinesin molecule at saturating ([ATPsat] = 1 mM) and limiting ([ATPlim] = 10 μM) ATP concentrations. The external load, calculated from the trap stiffness (0.05 pN nm−1), is indicated on the right.
