. 2016 Jul 13;153(2):341–351. doi: 10.1093/toxsci/kfw126

TABLE 1.

Comparison of the effect of flutamide, 2-hydroxyflutamide, and bicalutamide exposure on intracellular ATP content of HepG2 cells (2 h) in glucose and galactose medium

Compound	EC₅₀ATP (μM)		EC₅₀ATPglu/EC₅₀ATPgal (P-value)
Compound	Glucose	Galactose	EC₅₀ATPglu/EC₅₀ATPgal (P-value)
Flutamide	255	43.9	5.82 (0.03)
2-hydroxyflutamide	267	48.5	5.51 (0.01)
Bicalutamide	148	103	1.44 (0.22)

EC₅₀ATP refers to the concentration of the compound required to reduce cellular ATP content by 50%. Comparison of this value in cells treated in glucose or galactose medium identifies a mitochondrial liability if EC₅₀ATPglu/EC₅₀ATPgal ≥ 2. P-value indicates the significance of EC₅₀ATPglu compared with EC₅₀ATPgal.