FIG. 4.

Two-way hierarchical clustering dendrogram of drug-processing genes (DPGs) that were differentially expressed by pregnenolone-16α-carbonitrile (PCN) or 1,4-bis-[2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP) at day 3, A, or day 60, B, in the livers of wild-type (WT) mice. Three biological replicates (shown as columns) were tested for each group. Each row represents a different gene. A two-way hierarchical clustering dendrogram was generated of the differentially regulated DPGs by either PCN or TCPOBOP (fold-change > 2, P < 0.05) using the standardized means and Ward’s minimal variance. Distances between genes reflect the significance of associations. Red represents relatively higher and blue represents relatively lower expression. The expression profiles of differentially expressed drug-processing genes (DPGs) were categorized to 3 major patterns of interest at day 3, and two patterns at day 60.