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. 2016 Jul 13;153(2):282–302. doi: 10.1093/toxsci/kfw127

FIG. 5.

FIG. 5

Neonatal pregnane X receptor (PXR) activation caused acute (day 3) and persistent changes (day 60) in the expression of drug-processing genes (DPGs) in the livers of wild-type (WT) mice but not pregnane X receptor knockout mice (PXR-null). The microarray data of the drug-processing genes (DPGs) that indicated up-regulated (eg fold-change > 2, P < 0.05) or down-regulated (eg fold-change<-2, P < 0.05) by pregnenolone-16α-carbonitrile (PCN) at day 3, A, or day 60, B, in livers of wild-type (WT) mice were further validated by RT-qPCR in both WT and PXR-null mice. C, Western blot analysis for protein expression of Cyp4a14 in 60-day-old WT and PXR-null mouse livers. D, Cyp4a enzyme activity in 60-day-old WT and PXR-null mouse liver microsomes using the Promega P450-GloTM CYP4A Luciferin-4A Assay. Asterisk (*) indicates statistically significant differences between PCN and control group (P< 0.05) by Student’s t-test.