FIG. 7.

Chromatin immunoprecipitation (ChIP)-qPCR of the DNA binding fold enrichment for PPARα and RNA Pol II to Cyp4a gene loci in 60-day-old wild-type (WT), pregnane X receptor knockout mouse (PXR-null) and constitutive androstane receptor knockout mouse (CAR-null) livers. ChIP assays were performed using specific antibodies against peroxisome proliferator-activated receptor alpha (PPARα), RNA polymerase II (RNA Pol II), and Immunoglobulin G (IgG). Data were first normalized to genomic DNA input, and then expressed as fold-enrichment over IgG control.